Practicality of rRNA-gene-libraries for the characterization of biological agents in the air of work places

  • Project number: F 2289
  • Institution: Federal Institute for Occupational Safety and Health (BAuA)
  • Status: Completed Project

Description:

Molecular biological methods are currently used in many scientific studies to detect microorganisms qualitatively as well as quantitative, for examples in ecological studies or in medical diagnostics. For the detection of occupational exposure however, these methods have been used very rarely. With regard to risk assessment these methods can clearly increase our knowledge for occupational safety. The goal of this project is to verify whether the generation of 16S rRNA gene clone libraries from bioaerosol samples, are suitable to detect exposure to bacteria on highly contaminated workplaces with mixed exposures with high quality. Presumably this method has got the potential to be suitable for standardization.

Publications

Heterogeneity in Cultivation-Based Monitoring of Airborne Bacterial Biodiversity in Animal Farms

Publishing year: 2017

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Preliminary Validation of a Method Combining Cultivation and Cloning-Based Approaches to Monitor Airborne Bacteria

Publishing year: 2017

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Automated Image Analysis for Determination of Antibody Titers Against Occupational Bacterial Antigens Using Indirect Immunofluorescence

Publishing year: 2016

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Further Information

Contact

Unit 4.II.2 "Bioaerosols"

Phone: +49 231 9071-1971
Fax: +49 231 9071-2070