• Project number: F 2215
• Institution: Justus-Liebig Universität Gießen - Institut für Angewandte Mikrobiologie - IFZ
• Status: Completed Project

Description:

Thermoactinomyces vulgaris is a causative organism of hypersensitive pneumonitis. The cultivation based detection of this bacterial strain is very difficult because the phenotypic characteristica, depending on the cultivation conditions are highly variable. Therefore a strain specific primer system should be generated to gain a gen based detection system allowing the rapid and specific detection of Thermoactinomyces vulgaris with a quantitative real-time PCR in bioaerosols. Each analystical step of this method should be ckecked thoroughly.